HaloPlexHS – NEW
The detection of somatic variants in heterogeneous cell populations is crucial in a wide variety of applications such as cancer genetics and immunological diversity. Unlike inherited mutations, somatic variants often occur at low allele frequencies that require sensitive methods for detection. HaloPlexHS is a high sensitivity amplicon-based targeted sequencing method based on the HaloPlex technology that incorporates molecular barcodes in the DNA library, allowing for the identification of duplicate reads hence significantly improving base calling accuracy even at low allelic frequencies compared to conventional NGS methods. Combined with the flexibility of panel customization up to 5Mb and an accelerated workflow of less than 6hr starting with 50ng gDNA, HaloPlexHS is well-suited for accurate rare variant detection in clinical research.
- Uniquely tag DNA fragments with more than a million 10-nt molecular barcodes
- Confidently detect mutations present at below 1% allele frequency in genetically heterogeneous samples
- Differentiation of true variants from PCR or formalin fixation artifacts by targeting both DNA strands
- High on-target specificity and deep coverage of target bases so key variants are not missed
- Complete target enrichment in less than 6hr from only 50ng of gDNA
- From raw data to categorized mutations in 3 steps using SureCall data analysis software
To start your HaloPlexHS Illumina and ION custom design, use the SureDesign online tool
HaloPlexHS also provides molecular-barcoded target enrichment for catalog ClearSeq AML and ClearSeq Cancer Research panels. Learn more >
For Research Use Only. Not for Use in Diagnostic Procedures.